ROSELLINIA LIMONIISPORA: THE EFFECT OF BIOTIN AND THIAMIN AND VARIOUS SUGAR SOURCES UPON PERITHECIAL PRODUCTION IN PURE CULTURE

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Abstract
A nutritional study of Rosellinia limoniispora, a Pyrenomycete, was made on natural and defined media to ascertain some of the factors influencing the production of perithecia in pure culture. The importance of the vitamins biotin and thiamin was determined, together with the effects of different sugars tested singly and in combination. Dextrose was the best single sugar source for fruiting, but a medium containing a combination of four or five sugars produced most perithecia in as short a period of time as any other medium except 2% malt extract. On the latter the fruiting time was distinctly shorter than on any other medium and the number of perithecia formed was still high.