Structural Insights into the Function of the Thiamin Biosynthetic Enzyme Thi4 from Saccharomyces cerevisiae,
- 25 August 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 45 (37) , 11061-11070
- https://doi.org/10.1021/bi061025z
Abstract
The structure of thiazole synthase (Thi4) from Saccharomyces cerevisiae was determined to 1.8 Å resolution. Thi4 exists as an octamer with two monomers in the asymmetric unit. The structure reveals the presence of a tightly bound adenosine diphospho-5-(β-ethyl)-4-methylthiazole-2-carboxylic acid at the active site. The isolation of this reaction product identifies NAD as the most likely precursor and provides the first mechanistic insights into the biosynthesis of the thiamin thiazole in eukaryotes. Additionally, the Thi4 structure reveals the first protein structure with a GR2 domain that binds NAD instead of FAD, raising interesting questions about how this protein evolved from a flavoenzyme to a NAD binding enzyme.Keywords
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