RECONSTITUTION OF AMYLOID FIBRILS FROM ALKALINE EXTRACTS
Open Access
- 1 November 1967
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 35 (2) , 459-464
- https://doi.org/10.1083/jcb.35.2.459
Abstract
Amyloid fibrils were extracted with NH4OH and other solvents at high alkaline pH (pH 11.5), and then precipitated by reducing the pH to 4-5 with 1-2 N HCl. The precipitate showed tinctorial and ultrastructural properties identical with those of native amyloid. Attempts were also made to obtain this derivative in purer form by using a fractional extraction scheme. These methods are useful in the purification and reconstitution of the amyloid fibril and its sub-units.Keywords
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