A Technique for Preparing Ciliated Rumen Protozoa for Scanning Electron Microscopy

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Abstract
A rapid and efficient method of preparing ciliated rumen protozoa for scanning electron microscopy (SEM) was developed. Rumen contents were removed from a fistulated sheep and filtered through cheesecloth. The rumen fluid was then placed in a separatory funnel and incubated for 45 min at 39 C. The protozoal sludge which settled to the bottom of the flask was removed and fixed with glutaraldehyde. The fixed cells were collected on a membrane filter, washed with buffer, dehydrated in ethanol and then soaked in amyl acetate. The protozoa were subjected to critical point dehydration using CO2 as the intermediary fluid and then coated with gold or gold-palladium alloy before being viewed under the scanning electron microscope. Copyright © 1977. American Society of Animal Science . Copyright 1977 by American Society of Animal Science.

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