Enzymes of Heavy‐Metal‐Resistant and Non‐Resistant Populations of Silene cucubalus and Their Interaction with Some Heavy Metals in vitro and in vivo

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Abstract
The in vitro and in vivo effects of copper, zinc, cadmium, nickel, cobalt, and manganese on nitrate reductase, malate dehydro‐genase, isocitrate dehydrogenase, and glucose‐6‐phosphate dehydrogenase of zinc‐, copper‐ and non‐resistant populations of Silene cucubalus were investigated. During the in vitro experiments no resistant enzyme could be detected; enzymes of resistant and non‐resistant ecotypes had a similar sensibility to all the metals. Nitrate reductase was the most sensitive enzyme. During the in vivo experiments remarkable differences were found. The nitrate reductase and the isocitrate dehydrogenase of the zinc‐resistant population were activated when adding zinc to the culture medium, especially the nitrate reductase showed high activities at zinc concentrations where the nitrate reductase of the non‐zinc‐resistant populations was nearly completely inhibited. The zinc‐resistant ecotype had a real need for zinc.