Rat Hemoglobin Heterogeneity: Postnatal Changes in Proportions of Multiple Components and Effects of Erythropoietin on Marrow Cell Cultures

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Abstract
Hemolysate from adult and newborn rats was fractionated into 9 components (I-IX) using DEAE-cellulose chromatography. A subsequent chromatographic step resolved component IV into 2, IVa and IVb. A comparison of adult and newborn rat hemolysates showed that newborn had higher levels of components I, II and IV. In the newborn, they accounted for 52% of total Hb eluted, compared to 36% in the adult. Components V, VI and VIII were less in the newborn than in the adult red cell, at 42% compared to 59%. Minor components III, VII and IX were unchanged, at 5.8% in newborn and 5.3% in adult rats. Bone marrow cells from adult rats cultured for 46 h, showed stimulation of 8 components under the influence of erythropoietin (components IVa and IVb were not distinguished, and VII was not detected). The extent of stimulation was not consistently higher for major than for minor Hb. Adult rats possess at least 10 Hb components. Newborn rats have the same components but in different proportions. Both major and minor components are stimulated by erythropoietin in bone marrow cell cultures.
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