Abstract
Stomatal aperture in irrigated and non-irrigated plants of Coffea arabica L. was studied by measuring the infiltration into the leaf of mixtures of iso-propanol and distilled water. In pot experiments, stomatal aperture proved to be closely related to soil moisture content. In the field, the coefficient of the correlation between stomatal aperture and soil moisture content in the 10 ft deep rooted zone was 0.929 (r2 = 0.863) in the dry season and 0.773 (r2 = 0.598) in a period of intermittent rain. Stomatal aperture was invariably inversely correlated with the calculated soil moisture deficits of five irrigation treatments. Application of nitrogen fertilizer increased stomatal aperture but no explanation can be offered for this effect. Stomatal aperture as measured by this particular method was linearly correlated with temperature, vapour-pressure deficit and radiation, and also related to the time of day.

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