Abstract
About 50% of plantlets regenerated from dark-green islands of tobacco [Nicotiana tabacum] leaves systematically infected with tobacco mosaic virus were virus-free. An obvious advantage of using these virus-free areas is that they are visually distinguishable and can be rapidly excised and plated to initiate regeneration. Leaf sap from such virus-free plantlets apparently contains a factor which is inhibitory to TMV infection. These findings support the hypothesis of Atkinson and Matthews that green islands are delimited by an unidentified diffusible agent.

This publication has 2 references indexed in Scilit: