Isolation of immunogenic neuraminidases of human influenza viruses by a combination of genetic and biochemical procedures

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Abstract
Neuraminidases were purified from reassortant viruses (H6N1 and H6N2) containing the 2 antigenic subtypes (N1 and N2) found in human influenza viruses. Surface glycoproteins were solubilized with octylglucoside; the neuraminidase was isolated by chromatography on DEAE-Sephadex. Neuraminidase isolated by this technique coeluted with viral lipids and spontaneously formed liposomes on dialysis. The purified neuraminidase was immunogenic in rabbits, producing a significant antibody response at dose levels as low as 1 .mu.g.