Is alcoholic acidic silver nitrate reagent really specific for the histochemical localization of ascorbic acid?
- 1 February 1976
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 46 (2) , 173-175
- https://doi.org/10.1007/bf02462742
Abstract
The histochemical localization of ascorbic acid in plant tissues with the alcoholic acidic silver nitrate reagent is shown here to be not specific for ascorbic acid, since some of the polyphenolic substances, including flavonoids, which are known to be widely distributed in plant tissues, are also able to reduce the acidic alcoholic silver nitrate reagent at low temperature (0–4°C) and at pH 2 to 2.5 in dark. This method may perhaps be used for animal tissues where flavonoid pigments do not occur in such large quantities as they do in plants. I therefore, come to the inevitable conclusion that the use of alcoholic acidic silver nitrate reagent in localizing ascorbic acid in plant tissues may be highly misleading.This publication has 1 reference indexed in Scilit:
- On the specificity of the alcoholic, acidic silver nitrate reagent for the histochemical localization of ascorbic acidHistochemistry and Cell Biology, 1969