Molecular analysis of the region encoding the lytic system from Oenococcus oeni temperate bacteriophage φ10MC
- 15 February 1999
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 171 (2) , 231-238
- https://doi.org/10.1016/s0378-1097(99)00006-3
Abstract
Malolactic fermentation by Oenococcus oeni is a crucial step in wine-making. Oe. oeni phages are thought to be responsible for fermentation failures, yet they have received little attention. After a molecular analysis concerning the phage φ10MC integration system, this paper focuses on the lytic system. The att P (phage attachment site)-flanking region has been cloned and sequenced. The 1296-bp lysin gene ( Lys ) was identified in this region. The deduced amino acid sequence showed classical structural features of phage lysins, and this gene product expressed in Escherichia coli had a lytic activity against Oe. oeni . Downstream of Lys , a second ORF was present ( P163 ). According to its amino acid sequence and the location of its gene, the product could be the φ10MC holin. This study shows that the genomic organization of phage φ10MC att P-flanking regions is very similar to that of other lactic acid bacteriophages.Keywords
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