Response of Dioryctria amatella1 Males to Crude Pheromone Extracts in Laboratory Bioassays

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Abstract
The presence of a female sex pheromone in Dioryctria amatella (Hulst) was demonstrated and a bioassay procedure was developed for use in further studies. Male response to 5 concentrations of crude pheromone extract was determined. Male behavioral responses included orientation to the pheromone source, rapid circling or searching at the source, and wing fluttering. Orientation was the most commonly observed response with 90% of the males responding to 0.5 female equivalents of extract (FE). The percentage of males responding decreased slowly with decreasing concentration to 0.01 FE then-rapidly decreased at 0.005 FE. Low intensity red light enhanced male response, while low intensity white light or the presence of more than one male per test chamber reduced response.

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