In Situ Nuclear Magnetic Resonance of ¹⁵N Pulse Labels Monitors Different Routes for Nitrogen Assimilation

1 November 1992

journal article
Published by Oxford University Press (OUP) in Plant Physiology

Vol. 100 (3) , 1584-1586
https://doi.org/10.1104/pp.100.3.1584

Abstract

Nuclear magnetic resonance offers the possibility of noninvasive in situ observation of ¹⁵N pulse labeling in the presence of light. In vivo, exclusively the δ-nitrogen of Gln is labeled in the cyanobacterium Microcystis firma when glutamate synthase is inhibited by azaserine. In contrast, the green alga Chlorella fusca is additionally capable of incorporating nitrogen into Glu, thus providing evidence for an anabolic function of glutamate dehydrogenase in this organism.

Keywords

This publication has 3 references indexed in Scilit:

The Role of Glutamate Dehydrogenase in Plant Nitrogen Metabolism
Plant Physiology, 1991
A ¹⁴N and ¹⁵N Nuclear Magnetic Resonance Study of Nitrogen Metabolism in Shoot-Forming Cultures of White Spruce (Picea glauca) Buds
Plant Physiology, 1989
Alternative route for nitrogen assimilation in higher plants
Nature, 1974

In Situ Nuclear Magnetic Resonance of 15N Pulse Labels Monitors Different Routes for Nitrogen Assimilation

Abstract

Keywords

In Situ Nuclear Magnetic Resonance of ¹⁵N Pulse Labels Monitors Different Routes for Nitrogen Assimilation