Abstract
Experience with unfixed and fixed specimens has shown that there is no non-specific staining with the unfixed specimens, the controls remaining completely negative; with the fixed specimens, however, the controls show a certain amount of non-specific fluorescence. It is, however, possible to differentiate the test slides from the controls because of the difference in the intensity of the fluorescence. In terms of brightness, acetone gave the best results among the 5 different fixatives tried.