A simple technique for obtaining functionally isolated guard cells in epidermal strips of Vicia faba

Abstract
Ultrasonic irradiation of Vicia faba L. epidermal strips for 2 min preferentially disrupts the epidermal cells but does not impair guard cell movements. Maximal opening induced by fusicoccin requires that K+ be provided to the guard cells from external sources. A mobile organic anion is not required.