Mechanism of Spermine Stimulation of Rat-Liver Isoleucyl-tRNA Formation
Open Access
- 1 September 1978
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 90 (1) , 13-19
- https://doi.org/10.1111/j.1432-1033.1978.tb12569.x
Abstract
The mechanism of spermine stimulation of rat‐liver isoleucyl‐tRNA formation has been studied by Cleland's method [Biochim. Biophys. Acta 67, 104–137 & 173–197 (1963)]. It was demonstrated that isoleucyl‐tRNA formation proceeded through a ping‐pong mechanism in the presence of spermine and magnesium. From a study of the effect of spermine on both the formation of isoleucyl‐AMP · enzyme complex and on the subsequent formation of isoleucyl‐tRNA from the isoleucyl‐AMP · enzyme complex, evidence is presented that the stimulatory effect of spermine functions mainly through the spermine preventing the tRNA inhibition of isoleucyl‐AMP · enzyme complex formation.This publication has 24 references indexed in Scilit:
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