Affinity of integrin α1β1from liver sinusoidal membranes for type IV collagen
Open Access
- 19 August 1991
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 288 (1-2) , 241-243
- https://doi.org/10.1016/0014-5793(91)81044-9
Abstract
Hepatic sinusoidal membanes isolated from adult rats were extracted with detergent and fractionated on a wheat germ agglutinin affinity column. Bound glycoproteins were eluted with N-acetyl glucosamine and chromatographed on a type IV collagen affinity column. Recovery of the bound fraction by EDTA and analysis by SDS-PAGE revealed two glycoproteins with apparent molecular weights of 180 000 and 117 000. These were identified immunologically by Western blotting as the α and β subunits of integrin α1β1.Keywords
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