Inhibition of 2-acetylaminofluorene oxidations by monoclonal antibodies specific to 3-methylcholanthrene-induced rat liver cytochrome P450
- 1 January 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 4 (5) , 639-641
- https://doi.org/10.1093/carcin/4.5.639
Abstract
The inhibitory effects of ten monoclonal antibodies specific for methylcholanthrene (MC)-induced P 450 were determined on both C- and N-hydroxylations of 2-acetylaminofluorene (AAF) by liver microsomes from rats pretreated with MC, phenobarbital as well as untreated animals. The inhibitory effects of these antibodies were also determined on AAF hydroxylations by purified rat liver MC-P 450 . The inhibition patterns of AAF hydroxylations with both microsomes and the purified P 450 indicate that the formation of 7-OH-, 5-OH-, 3-OH-, 1-OH- and N-OH-AAF was catalyzed by the same unique isoenzyme or at least isoenzymes of common antigenic determinants.Keywords
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