Molecular Cloning and Characterization of a TAR-Binding Nuclear Factor from T Cells
- 1 June 1995
- journal article
- research article
- Published by Mary Ann Liebert Inc in AIDS Research and Human Retroviruses
- Vol. 11 (6) , 663-669
- https://doi.org/10.1089/aid.1995.11.663
Abstract
The Tat protein of the human immunodeficiency virus type 1 (HIV-1) activates the expression of viral mRNA through a cis-acting element in the LTR termed TAR. TAR RNA forms a stable stem-loop structure. Mutagenesis studies indicate that the stem structure, the primary sequence of the loop, and three unpaired bases in the stem (bulge) are important for Tat activation. Using the in vitro-transcribed TAR RNA as a probe, we have cloned a gene (TARBP-b) that encodes a TAR-binding protein from a cDNA expression library derived from Hut-78 cells. Expression of the 1.4-kb TARBP-b mRNA was observed in all mammalian cell lines tested. TARBP-b binds specifically to the bulge region of TAR RNA and trans-activates the HIV-1 long terminal repeat in the presence of ptat and prev expression plasmids. These results suggest that TARBP-b contributes to tat-mediated trans-activation.Keywords
This publication has 43 references indexed in Scilit:
- An Improved CAT Assay for Promoter Analysis in Either Transgenic Mice or Tissue Culture CellsDNA and Cell Biology, 1992
- Two distinct nuclear transcription factors recognize loop and bulge residues of the HIV-1 TAR RNA hairpin.Genes & Development, 1991
- tat regulates binding of the human immunodeficiency virus trans-activating region RNA loop-binding protein TRP-185.Genes & Development, 1991
- Characterization of a Human TAR RNA-Binding Protein That Activates the HIV-1 LTRScience, 1991
- A nuclear translational block imposed by the HIV-1 U3 region is relieved by the Tat-TAR interactionCell, 1990
- Trans-activation by HIV-1 Tat via a heterologous RNA binding proteinCell, 1990
- Activation of transcription by HIV-1 Tat protein tethered to nascent RNA through another proteinNature, 1990
- Retrovirus activation in embryonal carcinoma cells by cellular promoters.Genes & Development, 1989
- The trans-activator gene of the human T cell lymphotropic virus type III is required for replicationCell, 1986
- The primary structure of ribosomal protein L3 from Escherichia coli 70 S ribosomesFEBS Letters, 1978