The Effect of Temperature on the Rate of Conversion of p,p′-DDT to p,p′-DDE in Brook Trout (Salvelinus fontinalis)

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Abstract
Brook trout (Salvelinus fontinalis) were maintained in flowing fresh water at 2, 10, and 18 °C and injected intramuscularly with p,p′-[14C]DDT. Fish were killed at intervals up to 16 weeks after injection, and analyzed by a combination of thin-layer chromatography, gas-liquid chromatography, and liquid-scintillation counting. Only one labelled metabolite, p,p′-DDE, was detected during the experimental period. Its rate of production varied with temperature; after 16 weeks, about 9, 13, and 19% of the original p,p′-DDT in fish maintained at 2, 10, and 18 °C, respectively, had been converted.

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