SOME STUDIES ON MUCOSUBSTANCES OF BOVINE CORTICAL BONE

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Abstract
Bone powder, obtained by fragmentation of bovine cortical bone under cooling conditions, on extraction with sodium ethylenediaminetetraacetate (pH 7-8) gives four soluble products. The extracted substances fractionated electrophoreti-cally and by ion-exchange chromatography consist of a collagen-like fraction, a mucoprotein fraction, chondroitin sulphate and a sialoprotein. The sialoprotein is characterized by a high content of N-acetylneuraminic acid (15.9%) and hexoses (galactose, glucose and mannose) (13*6%), with fucose (2-4%) and amino sugars (made up of approximately equimolar proportions of glucosamine and galactosamine) (6-4%). The material contained traces only of uronic acid. In general properties, the sialo-protein resembles the orosomucoid of serum, but is distinguished from it at least by its content of galactosamine.