ADVANTAGE OF SPECTROPHOTOMETRY IN DERIVATIVE FOR THE DOSAGE PLASMA AND URINARY HEMOGLOBIN - COMPARISON WITH THE METHOD USING ALLEN CORRECTION

  • 1 January 1986
    • journal article
    • research article
    • Vol. 44  (3) , 242-248
Abstract
We are proposing a technique enabling for determining the concentration of hemoglobin in plasma or urine using spectrophotometry in derivative double dash. Around 420 nm hemoglobin has an elevated molecular extinction factor but the absorption of natural pigments creates a non-negligible interference in the classic spectrophotometric methods. On the contrary, the use of the derivative double dash practically eliminate the background noise and obtains a specific signal for hemoglobin while keeping an excellent sensitivity. In the plasma, where hemoglobin is entirely linked to haptoglobin, the concentration is determined by direct reading (while in the urine the presence of several pigment forms requires the use of Drabkin''s reagent. The method is simple, quick, precise and enables to titrate 1 mg.l (or 0.015 .mu.mol.l-1) of hemoglobin. The influence of bilirubin is negligible. The comparison with a method using Allen''s correction shows the superiority of the former as far as precision and accuracy are concerned, by avoiding the danger of a non-negligible systematic error.

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