Immobilization as a tool for the stabilization of lignin peroxidase produced byPhanerochaete chrysosporium INA-12
- 1 January 1993
- journal article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 38 (1-2) , 57-67
- https://doi.org/10.1007/bf02916412
Abstract
Lignin peroxidase immobilization was achieved by covalent coupling on CNBr-Sepharose 4B. Protein immobilization yield was around 80%. For veratryl alcohol oxidation, in the presence of hydrogen peroxide, both soluble and bound enzymes exhibited the same pH profile with an optimum near 2.5. Catalytic parameters (kc andK m ) were seriously affected by immobilization. On the other hand, immobilization provided a noticeable stabilization of the enzyme against acidic pH and high temperatures. A 15–20 increase in the half-inactivation times at pH 2.2 and 2.7, respectively, could be observed. Bound enzyme was also much more thermostable than soluble.Keywords
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