Improved High Performance Liquid Chromatographic Method for the Quantification of 3-Methylhistidine in Serum and Urine
- 1 June 1991
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 14 (9) , 1715-1728
- https://doi.org/10.1080/01483919108049649
Abstract
We optimized an high-performance liquid chromatographic (HPLC) method for the determination of 3-methylhistidine (3-MeHis) in biological fluids. After pre-column derivatization with OPA, analytical separation was achieved on a reversed-phase C18 column by a simple gradient between sodium propionate buffer and acetonitrile. the method is accurate, reproducible and sensitive, and allows the determination of urinary 3-MeHis levels in about 55 min. Other additional 16 amino acids may be easily quantified while the 3-MeHis peak is well resolved from an unknown urinary compound potentially interfering.Keywords
This publication has 17 references indexed in Scilit:
- Gas chromatographic quantitative determination of 1- and 3-methylhistidine in urine and muscles: comparison with glass capillary determinationJournal of Chromatography B: Biomedical Sciences and Applications, 1984
- Plasma levels of 3-methylhistidine after ingestion of the pure amino acid or of muscular proteins measured by radioimmunoassayMetabolism, 1984
- Quantitation of the urinary methylhistidine isomers by a combination of thin-layer and fluorometric techniquesJournal of Chromatography B: Biomedical Sciences and Applications, 1982
- Simple and rapid high-performance liquid chromatographic method for the quantification of 3-methylhistidineJournal of Chromatography B: Biomedical Sciences and Applications, 1981
- Picomole assay for Nτ-methylhistidine by gas chromatography—Mass spectrometryAnalytical Biochemistry, 1981
- Gas chromatographic separation of 3-methylhistidineJournal of Chromatography A, 1980
- Relationship between fat-free weight and urinary 3-methylhistidine excretion in manMetabolism, 1980
- Muscle protein breakdown rates in humans based on Nτ-methylhistidine (3-methylhistidine) content of mixed proteins in skeletal muscle and urinary output of Nτ-methylhistidineMetabolism, 1978
- Metabolism of 3-methylhistidine in manMetabolism, 1975
- 3-Methylhistidine, a component of actinBiochemical and Biophysical Research Communications, 1967