Regulation of nicotine biogenesis. 3. Biochemical basis of increased nicotine biogenesis by urea in tissue cultures of tobacco
- 1 December 1982
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Botany
- Vol. 60 (11) , 2371-2374
- https://doi.org/10.1139/b82-290
Abstract
Floral bud callus of Nicotiana tabacum L. var. Anand-2 could be grown on Murashige and Skoog medium with 10 mM urea as sole nitrogen source. Urea-treated tissues showed elevated nicotine content over the control. Activities of phenylalanine aminotransferase and tyrosine aminotransferase did not show close correspondence with growth or nicotine synthesis, whereas ornithine aminotransferase (OAT) activity exhibited correlation with nicotine biogenesis. Urea-treated callus showed higher OAT activity than the control. The activity of ornithine transcarbamylase (OTC) showed inverse correlation with nicotine synthesis. OTC and arginase activities were suppressed in urea-treated tissues. The implication of the results is discussed.This publication has 1 reference indexed in Scilit:
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962