The Development of Piroplasma canis in Culture

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Abstract
In the course of their investigations upon different species of Piroplasma numerous observers have kept infected blood in the ice-chest, or at room temperature, for various periods of time prior to the inoculation of animals. On the evidence afforded by microscopic examination alone some observers have concluded that the parasites are capable of considerable multiplication in defibrinated blood kept in vitro. Under these conditions many corpuscles undergo haemolysis and in consequence the parasites appear to be more numerous. We believe that this source of error explains the conclusions arrived at by Lignières and others with regard to the multiplication of P. bovis in extravascular blood (see p. 253).

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