The effects of cadmium on succinate and NADH‐linked substrate oxidations in rat hepatic mitochondria

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Abstract
Low concentrations of cadmium (3.3–40 μM) inhibited State 3 NADH‐linked respiration in rat hepatic mitochondria, but failed to release oligomycin (1 μM) inhibited State 3 respiration, or to significantly change the State 4 rate. In the presence of succinate, (40 μM) cadmium inhibited State 3 respiration by 89%, while concentrations between 3.3 and (13.3 μM) stimulated State 4 respiration. Higher concentrations caused marked inhibition. In the presence of succinate, cadmium released oligomycin inhibited State 3 respiration. Cadmium (0.001–1.0 mM) did not stimulate mitochondrial ATPase activity or inhibit ferricyanide reduction, but stimulated NAD+ linked mitochondrial dehydrogenase activities and NADH oxidation. These results indicate that cadmium interacts with either the NADH dehydrogenase complex or other NADH‐dependent enzymes and solely by an uncompling action.