The Translocation of Antibiotics in Higher Plants

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Abstract
Griseofulvin and chloramphenicol have been recovered from the tissues of broad-bean plants grown in solutions containing these antibiotics. Partial separation of the compounds from other constituents of solvent extracts of plant tissue was effected by counter-current distribution in a carbon tetra chloride methanol : water system. Pure griseofulvin and chloramphenicol were then obtained by recovery of the solute in appropriate tubes followed by chromatography on an activated alumina column; they were identified by mixed melting point determinations and comparison of their infra-red spectra. A method for estimating griseofulvin in plant tissue extracts based on counter current distribution and examination of the fractions spectrophotometrically has been developed, and used to strike a balance between the gnseofulvin entering the plant and that present in the tissues after treatment. Estimates of chloramphenicol in the tissues by bioassay agree well with the chemical determinations.

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