IDENTIFICATION OF ANAEROBES ON THE MINITEK SYSTEM, COMPARED TO A CONVENTIONAL SYSTEM

Google Scholar
  • 1 January 1979
    • journal article
    • research article
    • Vol. 245  (3) , 324-331
Abstract
The Minitek Miniaturized System (BBL) was compared to a conventional method (V.P.I.) for the identification of anaerobic bacteria. Anaerobiosis was achieved by using the GasPak system (BBL) and a glove box. Gram staining, colonial morphology, GLC and biochemical reactions as detailed in the Virginia Polytechnic Institute anaerobe laboratory manual were compared to the biochemical results obtained on the Minitek system. A total of 32 anaerobes was used. The percentage of correlation was an overall 98.2%. Discrepancies were found mostly in the production of indole, the reduction of nitrate and the hydrolysis of esculin. A further 95 anaerobes were tested on these 3 reactions and gave an overall agreement of 92.9%. It was necessary to use some additional tests and as GLC for definitive identification. The microsystem was also evaluated as to time saved, cost and ease of use.