Accumulation of Radioiodine (I131) in Thyroid Gland of the Hamster Embryo.

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Abstract
Injns. of radioactive NaI were made into the peritoneal cavity of the gestating hamster from the 8th to the 15th day after copulation. The doses of radioiodine ranged from 50 to 100 uc. Embryos which ranged from 8 to 15 days of development were obtained, fixed in an alcohol-formalin soln. (method B of Holt, Cowing and Warren) and in Bouin''s soln., after which they were washed in alcohol. Paraffin sections prepared from these embryos were mounted on microscope slides. Dental X-ray films were exposed to these sections for 6 and 8 days, depending upon the activity of the iodine. The sections were then stained with Delafield''s hematoxylin and eosin for microscopic study. A study of autoradiographs and stained sections revealed the first evidence of I-containing colloid on the 13th day of development. A distinct correlation between the differentiation of follicles and the storage of colloid was found, since colloid appeared only in the discrete follicle. Number of follicles present and amt. of colloid stored increased with the age of the embryo.

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