Inhibition of platelet phospholipase-A2 as a mechanism for the anti-aggregating effect of linoleic acid
- 1 April 1980
- journal article
- research article
- Published by Springer Nature in Inflammation Research
- Vol. 10 (1-2) , 57-62
- https://doi.org/10.1007/bf02024179
Abstract
Linoleic acid was incorporated into platelet phospholipids and then released after activation of phospholipase A2 (PL-A2) with thrombin or ionophore A23187. The rate of this release was tenfold lower for linoleic than for arachidonic acid. This observation strongly suggests that incorporation of linoleic acid in platelet phospholipids might inhibit platelet PL-A2 and might explain the anti-aggregating effect of linoleic acid. In fact it has also been shown that linoleic acid inhibits PL-A2 activity at concentrations which antagonize platelet aggregation. Moreover, in experimental conditions where platelet cyclo-oxygenase is totally inhibited, aggregation does occur and can still be blocked by linoleic acid. This latter observation led to the conclusion that the anti-aggregating effect of linoleic acid is independent from prostaglandin pathway and is probably related to the phospholipid metabolism.This publication has 19 references indexed in Scilit:
- The role of platelet-activating factor in platelet aggregationNature, 1979
- Structural analysis of purified platelet-activating factor by lipasesNature, 1977
- Carrageenan and thrombin trigger prostaglandin synthetase-independent aggregation of rabbit platelets: inhibition by phospholipase A2 inhibitorsJournal of Pharmacy and Pharmacology, 1977
- The mobilization of arachidonic acid in platelets exposed to thrombin or ionophore A23187. Effects of adenosine triphosphate deprivation.Journal of Clinical Investigation, 1977
- Selective release of archidonic acid from the phospholipids of human platelets in response to thrombin.Journal of Clinical Investigation, 1977
- Initiation of phospholipase A2 activity in human platelets by the calcium ion ionophore A23187Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1977
- Rat platelets aggregate in the absence of endogenous precursors of prostaglandin endoperoxidesNature, 1976
- Metabolism of [14C]arachidonic acid by human plateletsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1976
- Inhibition of prostaglandin synthesis by oleic, linoieic and linoienic acidsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1968
- Aggregation of Blood Platelets by Adenosine Diphosphate and its ReversalNature, 1962