Use of a centromere-specific DNA probe (p82H) in nonisotopic in situ hybridization for classification of micronuclei
- 1 May 1990
- journal article
- research article
- Published by Wiley in Genes, Chromosomes and Cancer
- Vol. 2 (1) , 59-62
- https://doi.org/10.1002/gcc.2870020111
Abstract
The DNA probe p82H was used to visualize centromeric DNA in micronuclei (MN) of human cells. Slides prepared from cultures treated by the aneugen (causing aneuploidy) colcemid showed significantly more MN with centromeric signals than those treated by the clastogen (causing chromosome breakage) bleomycin. These results indicate that in situ hybridization with the alphoid p82H DNA probe is a suitable method with which to distinguish between MN containing whole chromosomes and acentric fragments, and hence allows one to discriminate between the clastogenic and aneugenic effects in MN formation.Keywords
This publication has 13 references indexed in Scilit:
- A rapid boiling method for the preparation of bacterial plasmidsPublished by Elsevier ,2004
- The identification of micronucleated chromosomes: a possible assay for aneuploidyMutagenesis, 1988
- Chromosomal insertion of human papillomavirus 18 sequences in HeLa cells detected by nonisotopic in situ hybridization and reflection contrast microscopyHuman Genetics, 1987
- p82H identifies sequences at every human centromereHuman Genetics, 1987
- Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.Proceedings of the National Academy of Sciences, 1986
- Sequence of centromere separation: kinetochore formation in induced laggards and micronucleiMutagenesis, 1986
- Measurement of micronuclei in lymphocytesMutation Research/Environmental Mutagenesis and Related Subjects, 1985
- A simple method of reducing the fading of immunofluorescence during microscopyJournal of Immunological Methods, 1981
- A comparison of diameters of micronuclei induced by clastogens and by spindle poisonsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1980