A Study of the Immunological and Biological Properties of a Follicle-Stimulating Hormone

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Abstract
Two male rabbits were immunized with NIH FSH and the immunological properties of the antiserum were studied by means of agar gel diffusion and agar and starch immunoelectrophoresis. The 2 antisera absorbed with a variety of sheep tissues including sheep LH revealed 2 antigenantibody systems when reacted against the hormone. Each system formed a characteristic precipitin band. One of the 2 bands was identical in both sera, but the second differed. On the other hand, the hemagglutination titers were the same for the 2 antisera. A variety of biological assays in normal and hypophysectomized rats revealed that antiserum from the 2 rabbits were different with respect to antihormone potency, as indicated by organ weights and the histological differences. With these facts in mind, it was possible to postulate which of the 2 precipitin bands was the likely site of anti-FSH activity. The anti- FSH sera clearly inhibited the effect of mouse pituitary saline extracts on the assay rat ovary. (Endocrinology74: 314, 1964)

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