Differential stability of c-myc mRNAS in a cell-free system.
Open Access
- 1 July 1988
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 8 (7) , 2860-2868
- https://doi.org/10.1128/mcb.8.7.2860
Abstract
We have developed a simple cell-free system for studying the stability of different mRNAs in vitro. We demonstrate that the threefold greater stability in vivo of truncated c-myc mRNA (lacking exon 1) compared with that of full-length c-myc mRNA is maintained in our in vitro system. Chimeric mRNAs in which the first exon of c-myc was fused to immunoglobulin C alpha heavy chain or glyceraldehyde-3-phosphate dehydrogenase mRNAs were not rapidly degraded, demonstrating that c-myc exon 1 alone is not sufficient to tag mRNAs for rapid degradation. Competition experiments show that full-length c-myc mRNA is specifically recognized by a factor(s) responsible for its rapid degradation. This system will allow further characterization and purification of these factors.This publication has 42 references indexed in Scilit:
- A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradationCell, 1986
- Preparation of capped RNA transcripts using T7 RNA polymeraseNucleic Acids Research, 1986
- Transcriptional and posttranscriptional control of c-myc during myogenesis: its mRNA remains inducible in differentiated cells and does not suppress the differentiated phenotype.Molecular and Cellular Biology, 1986
- H4 histone messenger RNA decay in cell-free extracts initiates at or near the 3′ terminus and proceeds 3′ to 5′Journal of Molecular Biology, 1986
- Truncation of exon 1 from the c-myc gene results in prolonged c-myc mRNa stability.The EMBO Journal, 1985
- Post-transcriptional control of myc and p53 expression during differentiation of the embryonal carcinoma cell line F9Nature, 1985
- Posttranscriptional mechanisms are responsible for accumulation of truncated c-myc RNAs in murine plasma cell tumorsCell, 1985
- Mechanisms that Regulate Immunoglobulin Gene ExpressionAnnual Review of Immunology, 1985
- The effect of capping and polyadenylation on the stability, movement and translation of synthetic messenger RNAs inXenopusoocytesNucleic Acids Research, 1985
- Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoterNucleic Acids Research, 1984