Theoretical Foundations of Liquid Adsorption Chromatography with Mixed Eluent

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Abstract
One of the main advantages of liquid-solid (adsorption) chromatography (LSC) with mixed eluent is the possibility of modification of the capacity ratio over a wide range by changing the eluent composition [1]. It means that the resolution and the analysis time may be optimized by correct choice of the eluent composition (isocratic LSC) or by eluent programing during the chromatographic process (gradient LSC). Optimization of the chromatographic process requires of the knowledge of dependence of the chromatographic quantities on the eluent composition [2–5]. Increasing interest in gradient LSC [2–13] has created a pressing need for the elucidation of the theoretical basis of LSC with mixed eluent.

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