Detection of Francisella tularensis in blood by polymerase chain reaction
- 1 January 1993
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 31 (1) , 152-4
- https://doi.org/10.1128/jcm.31.1.152-154.1993
Abstract
We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/microliter of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.Keywords
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