Improved High-Performance Liquid Chromatographic (HPLC) Assay Method for Ceftizoxime
- 1 May 1984
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 7 (5) , 961-968
- https://doi.org/10.1080/01483918408074018
Abstract
A high-performance liquid chromatographic method was improved for the quantitative determiation of ceftizoxime in human serum and urine using cefotaxime as internal standard. It employed a .mu. Bondapak Alkyl Phenyl column, elution with acetonitrile-PO43- buffer and measurement of UV absorption at 254 nm. Results obtained using the HPLC assay were compared to those obtained using a microbiological [Bacillus subtilis] assay. The correlation coefficient was 0.987. The method was rapid, accurate and reproducible with a sensitivity of 2.5 .mu.g/ml of ceftizoxime. Cefotaxime and its major metabolite, the desacetylcefotaxime, were also quantitated by this procedure.This publication has 6 references indexed in Scilit:
- Ceftizoxime kinetics and renal handlingClinical Pharmacology & Therapeutics, 1982
- Phase I Study of Ceftizoxime, a New Cephalosporin. Single‐Dose StudyThe Journal of Clinical Pharmacology, 1981
- HPLC in clinical microbiology laboratoriesJournal of Antimicrobial Chemotherapy, 1981
- High-performance liquid chromatographic determination of ceftizoxime, a new cephalosporin antibiotic, in rat serum, bile and urineJournal of Chromatography B: Biomedical Sciences and Applications, 1980
- High-Pressure Liquid Chromatography for Quantitation of Antimicrobial AgentsClinical Infectious Diseases, 1980
- Comparative Serum Levels and Protective Activity of Parenterally Administered Cephalosporins in Experimental AnimalsAntimicrobial Agents and Chemotherapy, 1974