Isolation and Sequence Analysis ofrep Afrom the Incurable 90 MDA Plasmid ofAzospirillum Brasilense
- 1 January 2000
- journal article
- research article
- Published by Taylor & Francis in DNA Sequence
- Vol. 11 (1-2) , 101-107
- https://doi.org/10.3109/10425170009033976
Abstract
An open reading frame encoding a protein of 312 amino acids was identified in an essential region of the 90MDa plasmid of Azospirillum brasilense. The putative protein shows similarity with RepA proteins of plasmids of Sphingomonas aromacitovorans, Rhodoter-mus marinus, Chlorobium limicola and Bifidobacterium asteroides and with the RepA protein of the broad host range plasmid pSa. A putative helix-turn-helix motif was identified in the central part of the A. brasilense RepA protein. Analysis of the upstream and downstream region of the A. brasilense rep A gene revealed the presence of eight 15-bp repeats, which can possibly function as iterons.Keywords
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