Parallel, high-throughput purification of recombinant antibodies for in vivo cell assays
- 5 August 2006
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 94 (5) , 931-937
- https://doi.org/10.1002/bit.20914
Abstract
We describe a method for high‐throughput, parallel purification of secreted proteins to analyse large numbers of protein samples in cell‐based assays for the discovery of protein therapeutics. The procedure is generic and capable of 96 parallel purifications and compatible, in both yield and purity, with a wide assay range. By optimising expression and purification steps as well as using novel hardware, in particular a chromatography press capable to purify target proteins from viscous media, we exemplify the process for the generation of single‐chain Fv antibody fragments (scFv) and the purification of full‐length IgG. The described process can operate robustly with a throughput of over 2,000 samples per month.Keywords
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