Microchip Flow Cytometry Using Electrokinetic Focusing
- 7 August 1999
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 71 (19) , 4173-4177
- https://doi.org/10.1021/ac990372u
Abstract
Flow cytometry of fluorescently labeled and unlabeled latex particles is demonstrated on a microfabricated device. The latex particles were detected and counted using laser light scattering and fluorescence coincidence measurements. Sample confinement was accomplished using electrokinetic focusing at a cross intersection, and detection occurred 50 μm downstream from the intersection. Particles with diameters of 1 and 2 μm were analyzed and distinguished from each other based on their light scattering intensity and fluorescence. A maximum sample throughput of 34 particles/s was achieved. Sample mixtures with varying proportions of fluorescently labeled and unlabeled particles were also analyzed and found to be within experimental error of the expected ratios.Keywords
This publication has 17 references indexed in Scilit:
- Modular concept of a laboratory on a chip for chemical and biochemical analysisBiosensors and Bioelectronics, 1998
- Integrated Capillary Electrophoresis Devices with an Efficient Postcolumn Reactor in Planar Quartz and Glass ChipsAnalytical Chemistry, 1996
- Functional Integration of PCR Amplification and Capillary Electrophoresis in a Microfabricated DNA Analysis DeviceAnalytical Chemistry, 1996
- Integrated Microdevice for DNA Restriction Fragment AnalysisAnalytical Chemistry, 1996
- Ultra-High-Speed DNA Sequencing Using Capillary Electrophoresis ChipsAnalytical Chemistry, 1995
- High-Speed Separation of Antisense Oligonucleotides on a Micromachined Capillary Electrophoresis DeviceAnalytical Chemistry, 1994
- Continuous Sample Pretreatment Using a Free-Flow Electrophoresis Device Integrated onto a Silicon ChipAnalytical Chemistry, 1994
- Flow cytometryAnalytical Chemistry, 1994
- Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a ChipScience, 1993
- Planar glass chips for capillary electrophoresis: repetitive sample injection, quantitation, and separation efficiencyAnalytical Chemistry, 1993