Comparison of serotonin and 5-methoxytryptamine immunoreactivity in rat raphe nuclei
- 1 January 1986
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 85 (3) , 259-263
- https://doi.org/10.1007/bf00494813
Abstract
We studied the immunoreactivity of 5-methoxytryptamine (MT) and 5-hydroxytryptamine (HT) in the raphe region of rats using specific polyclonal antibodies and the peroxidase/anti-peroxidase (PAP) technique. Overall, the patterns of the specific staining for these two antibodies were found to be the same in this region of the rat brain. The staining reaction was considerably less intense for MT than for HT. Specificity tests were performed using HT, MT and tryptamine (T) conjugates at concentrations of 5×10−8 M for antibodies to HT and 2.5×10−9 M for antibodies to MT. Although the distribution of HT-like and MT-like immunoreactivity broadly overlapped, the results obtained from adsorption-specificity tests confirmed the presence of specific MT staining in the rat raphe.Keywords
This publication has 19 references indexed in Scilit:
- Specific detection of noradrenaline in the rat brain by using antibodiesBrain Research, 1986
- Tryptamine Concentrations in Areas of 5‐Hydroxytryptamine Terminal Innervation After Electrolytic Lesions of Midbrain Raphe NucleiJournal of Neurochemistry, 1985
- Immunocytochemical Detection of Acetylcholine in the Rat Central Nervous SystemScience, 1985
- Antisera Against the Indolealkylamines: Tryptophan, 5‐Hydroxytryptophan, 5‐Hydroxytryptamine, 5‐Methoxytryptophan, and 5‐Methoxytryptamine Tested by an Enzyme‐Linked Immunosorbent Assay MethodJournal of Neurochemistry, 1985
- Antisera against catecholamines: Specificity studies and physicochemical data for anti-dopamine and anti-p-tyramine antibodiesMolecular Immunology, 1984
- An immunohistochemical method for the localization of N-acetylserotonin (NAS) in the central nervous system. Description, validation, and application of the technique.Journal of Histochemistry & Cytochemistry, 1983
- Factors Influencing Melatonin, 5-Hydroxytryptophol, 5-Hydroxyindoleacetic Acid, 5-Hydroxytryptamine and Tryptophan in Rat Pineal GlandsNeuroendocrinology, 1982
- THE FLUOROMETRIC DETERMINATION OF 5‐METHOXYTRYPTAMINE IN MAMMALIAN TISSUES AND FLUIDSJournal of Neurochemistry, 1978
- EFFECTS OF LESIONS AND DRUGS ON BRAIN TRYPTAMINEJournal of Neurochemistry, 1974
- Classification of monoamine neurones in the rat mesencephalon: Distribution of a new monoamine neurone systemBrain Research, 1971