The transcriptional regulation of human arachidonate 12‐lipoxygenase gene by NFκB/Rel
Open Access
- 17 April 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 363 (1-2) , 105-110
- https://doi.org/10.1016/0014-5793(95)00293-i
Abstract
As examined by the luciferase assay, a negative regulatory region including the NFκB motif was found in the 5′‐flanking region of the 12‐lipoxygenase gene in human erythroleukemia cells. The negative control was abolished by a site‐specific mutation of the NFκB motif. Probes including the NFκB region gave positive bands upon a gel‐shift assay. The bands were supershifted by antibodies for NFκB p50, NFκB p65 and c‐Rel, and were lost by a NFκB competitor DNA. Furthermore, the NFκB sequence was protected in DNase I footprinting. Thus, two kinds of heterodimer (p50 and p65; p50 and c‐Rel) seemed to control the over‐expression of the human 12‐lipoxygenase gene.Keywords
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