CHANGES IN IMMUNOCOMPETENCE AND SURFACE MARKERS OF LYMPHOCYTES IN STORED-BLOOD

  • 1 January 1977
    • journal article
    • research article
    • Vol. 5  (1) , 8-12
Abstract
Changes in [human] lymphocyte responses to mitogens and other lymphocyte markers were studied during a 14-day period of storage of blood at 4.degree. C and 22.degree. C in plastic bags. The following tests were done: absolute lymphocyte and neutrophil counts; blastogenic response to PHA [phytohemagglutinin], PWM [pokeweed mitogen], Con-A [concanavalin A] and [Escherichia coli] LPS [lipopolysaccharide]; and determination of T [thymus-derived] and B [bone marrow-derived] cells using E[erythrocyte]-rosette and EAC[E, antibody, complement]-rosette techniques. The neutrophils disappeared rapidly at 22.degree. C. The absolute lymphocyte count doubled in a week. The responses to PHA, PWM and Con-A declined rapidly and were absent by the 6th day. The response to LPS disappeared on day 14. The ability to form E-rosettes and EAC-rosettes was lost by days 4 and 6, respectively. The results obtained at 4.degree. C were similar except that these changes were delayed varying from 1-7 days.

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