Comparison of emm Typing and Ribotyping with Three Restriction Enzymes To Characterize Clinical Isolates of Streptococcus pyogenes
Open Access
- 1 January 2005
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 43 (1) , 150-155
- https://doi.org/10.1128/jcm.43.1.150-155.2005
Abstract
A total of 336 Streptococcus pyogenes isolates recently recovered from patients with pharyngitis from 13 countries were characterized by emm typing and riboprinting using an automated Riboprinter (Dupont/Qualicon) based on the patterns produced by three restriction enzymes, EcoRI, PstI, and HindIII. Three enzymes were necessary to increase the discrimination of ribogroups formed by each enzyme. A total of 40 ribogroups and 38 emm sequences (not counting allelic variations) were identified. Multilocus sequence typing was performed on a sampling of the isolates, and those results were consistent with those of both emm typing and ribotyping. Correlations were observed among all three methods.Keywords
This publication has 10 references indexed in Scilit:
- Epidemiology of macrolide and/or lincosamide resistant Streptococcus pneumoniae clinical isolates with ribosomal mutationsDiagnostic Microbiology and Infectious Disease, 2004
- Clonal Relatedness of Erythromycin-Resistant Streptococcus pyogenes Isolates in GermanyAntimicrobial Agents and Chemotherapy, 2004
- Group A Streptococci from a Remote Community Have Novel Multilocus Genotypes but ShareemmTypes and Housekeeping Alleles with Isolates from Worldwide SourcesThe Journal of Infectious Diseases, 2004
- Multi-locus sequence typing: a tool for global epidemiologyTrends in Microbiology, 2003
- Comparison of In Vitro Activities of ABT-773 and Telithromycin against Macrolide-Susceptible and -Resistant Streptococci and StaphylococciAntimicrobial Agents and Chemotherapy, 2002
- Multilocus Sequence Typing ofStreptococcus pyogenesand the Relationships betweenemmType and CloneInfection and Immunity, 2001
- Comparative evaluation of an automated ribotyping instrument versus pulsed-field gel electrophoresis for epidemiological investigation of clinical isolates of bacteriaDiagnostic Microbiology and Infectious Disease, 1999
- emm Typing and Validation of Provisional M Types for Group A Streptococci1Emerging Infectious Diseases, 1999
- Multilocus sequence typing: A portable approach to the identification of clones within populations of pathogenic microorganismsProceedings of the National Academy of Sciences, 1998
- Sequencing emm-specific PCR products for routine and accurate typing of group A streptococciJournal of Clinical Microbiology, 1996