Studies on β-Lactam Antibiotics for Medicinal Purpose. IV. Stability of 6-[D (-)-α-(4-Ethyl-2, 3-dioxo-1-piperazinecarboxamido) phenylacetamido] penicillanic Acid (T-1220) in Aqueous Solution and Body Fluid

Abstract
The degradation pattern of 6-[D(-)-.alpha.-(4-ethyl-2,3-dioxo-1-piperazinecarboxamido)phenylacetamido]penicillanic acid (T-1220) in aqueous solution was investigated. In acidic solution, T-1220B and T-1220D were found, while T-1220A, T-1220B, and T-1220C were identified in alkaline solution. Stability of T-1220 in aqueous solution and body fluid was measured by means of high pressure liquid chromatography. T-1220 was more stable at neutral pH of 5.0-6.0, slightly unstable at acidic pH of 4.0 and markedly unstable at alkaline pH of 9.0. Stability of T-1220 in various body fluids seemed to depend on pH and its degradation pattern was similar to that in aqueous solution.

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