Liquid Nitrogen Cryo-Impacting: a New Concept for Cell Disruption
- 1 January 1975
- journal article
- research article
- Published by American Society for Microbiology in Applied Microbiology
- Vol. 30 (3) , 445-449
- https://doi.org/10.1128/aem.30.3.445-449.1975
Abstract
High-efficiency disruption of bacteria can be accomplished in 2 or more min by the new procedure of liquid nitrogen cryo-impacting. Release of the dipicolinic acid-Ca2+ chelate paralleled the breakage of Bacillus megaterium endospores. Lactate dehydrogenase activity was much better in supernates from liquid nitrogen cryo-impacting-broken Escherichia coli cells than in those from sonically treated and broken E. coli cells. ImagesKeywords
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