Isolation of a polysaccharide from the inner cell wall, intine, of pollen of Cryptomeria japonica

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Abstract
Isolation and solubilization of the intine of pollen grains of Cryptomeria japonica were attempted. The intine was separated effectively from other cell fragments by passing it through a column of glass beads and dissolving it in an EDTA solution. The extract was fractionated by column chromatography on DEAE-cellulose and gelfiltration through Sephadex G-200, and a polysaccharide component was obtained. This was found to consist of galactose, rhamnose, arabinose, xylose and uronic acid.