A Low-Resolution Separation of C14-Glyburide and Its Metabolites from Plasma Extracts Using a High-Performance Liquid Chromatograph Guard Column

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Abstract
A low-resolution method for simultaneous, rapid determination of radiolabeled glyburide [a hypoglycemic drug] and its metabolites in human plasma is described. Plasma samples were extracted with ethyl acetate. Extracts were redissolved in 300 .mu.l of mobile phase, and injected into a 3 cm guard column, which was incorporated as a loop in a 6-port switching valve. C14-glyburide was collected as a single 4-min fraction at a flow rate of 41 /min. Following collection of a 1-ml fraction, the column was backflushed with methanol to allow collection of a metabolites of glyburide. The mean value of recovered radioactivity was 95.5 .+-. 5.7%. The validity of the separation was verified in a high-resolution HPLC [high-performance liquid chromatography] system and no cross contamination of the fractions was observed.

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