Immunochemical Quantitation of Pepsin and Pepsinogen

Publisher Website
Google Scholar
Abstract
The immunochemical quantitation of the gastric and urinary pepsin and pepsinogen described here are based on the measurement of immunoprecipitations of the constituents in a gel in which one has previously incorporated a specific antibody against these proteases. The comparative studies by both electrophoretic immunodiffusion and by radial immunodiffusion have shown significant correlations and are sufficiently reproducible. Urine The existence of common antigenic determinants established for the principal human pepsin PII and one of the gastric proteases of pig permits the use of standard references and of specific antibodies of human or pig origin. In the course of gastro-duodenal ulcers with hypersecretion and hyperchlorhydria the increase of the absolute quantity of pepsin in the histamine secretion is confirmed. The absence of gastric pepsin and uropepsinogen has been verified in the course of certain gastric achlorhydrias with atrophy of the mucosa. In spite of certain differences established after utilization of several lots of commercially purified pepsin as references, the method of immunochemical quantitation of pepsin will permit a good international standardization which is impossible with the old method of measuring the proteolitic activities.

This publication has 0 references indexed in Scilit: