A DISRUPTION AND FRACTIONATION OF BOVINE EPIDIDYMAL SPERMATOZOA
- 1 February 1968
- journal article
- research article
- Published by Bioscientifica in Reproduction
- Vol. 15 (1) , 113-119
- https://doi.org/10.1530/jrf.0.0150113
Abstract
The colloid mill was found capable of disrupting bovine epididymal spermatozoa in such a way that even the very resistant sperm middle piece was opened. The homogenate thus produced was fractionated by both differential centri-fugation and isopyenic density gradient centrifugation. The latter method was found very useful in producing homogenous fractions of cell components including in order of increasing density: mitochondrial fragments, acrosomes, membranes, tail principal pieces, middle pieces, middle piece exteriors, contractile fibres and denuded heads. The mitochondrial particles consumed O2 while the cell membranes had hexokinase-like activity.This publication has 7 references indexed in Scilit:
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